The Kinetic HDAC1 BioAssay™ Kit is a complete assay system designed to measure histone deacetylase 1 (HDAC1) activity kinetically for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC1 activity measurements. In addition, the kit includes purified HDAC1 enzyme and a potent HDAC inhibitor, SAHA, for use as a positive and negative control. The Kinetic HDAC1 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only one simple step for setting up the HDAC1 reaction on a microtiter plate is needed and then the HDAC1 activity level can be measured kinetically using a fluorescence reader.
Applications:Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovry and HTS applications.
Developer Dilution Buffer, 1x10ml SAHA (1mM), 1x20ul HDAC Assay Buffer, 1x20ml BSA (1mg/ml), 1x1mlblack, low binding NUNC black microtiter plate, 1x1plate
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
