Fusion protein corresponding to the serine protease NS3 (a.a.3-181) and cofactor NS4A (a.a.21-32) from Hepatitis C virus genotype 1b (GenBank Accession No. AF054247) with Asp-toVal mutation on a.a.168, and N-terminal Flag-His tag, MW=22.3kD, expressed in an E. coli expression system.
Source:Recombinant protein corresponding to HCV1b at N-terminus, fused to Flag-His tag expressed in E.coli.
Molecular Weight:~22.3kD
Specific Activity:45pmole/min/ug
Application:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size:20ug
Source Antigen:Recombinant, E.coli
Grade:Highly Purified
Purity:~90%
Form:Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 400mM NaCl, 0.2% Triton X-100, 10mM b-mercaptoethanol, 0.1mg/ml Flag peptide, 30% glycerol.