The GCN5 Chemiluminescent BioAssay™ Kit is an enzyme-linked immunosorbent assay (ELISA) designed to screen for inhibitors of GCN5. Histone acetyltransferase GCN5 (also known as KAT2A) is involved in various cellular events, and its dysfunction is linked to a number of human diseases including cancers and diabetes. The GCN5 Chemiluminescent Assay Kit comes in a convenient format, with a 96-well plate precoated with Neutravidin and Histone peptide, and all the reagents necessary for 96 chemiluminescent GCN5 activity measurements. In addition, the kit includes purified GCN5 for use as a positive control. The GCN5 Chemiluminescent Assay Kit is based on the GCN5 enzyme transferring an acetyl group from an acetyl donor (acetyl CoA) to a histone substrate. The acetylated histone is recognized by a highly specific primary antibody, followed by an HRP-labeled secondary antibody. The chemiluminescence produced by HRP can be measured using a chemiluminescence reader.
Applications:Great for studying enzyme activity and screening small molecular inhibitors for drug discovery and HTS applications.
Components:GCN5 human recombinant enzyme, 1x20ugAcetyl CoA (1mM), 1x500ul10X HAT assay buffer, 1x10mlPrimary antibody 21, 1x50ulSecondary antibody 2, 1x12ulHRP chemiluminescent substrate A (transparent bottle)HRP chemiluminescent substrate B (brown bottle), 1x6mlBlocking buffer, 1x50mlWhite microplate (or strips) precoated with Neutravidin and Histone peptide
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
