Human FYN A (GenBank Accession No. NM_002037), full length with N-terminal Flag tag, a.a 2-537(end), MW= 61kD, expressed in Sf9 insect cells.
Source:Full length recombinant protein corresponding to aa2-537 from human FYN A at N-terminal, fused to Flag-tag expressed in Sf9 insect cells.
Molecular Weight:~61kD
Specific Activity:200mole/min/ug.
Assay Conditions:Enzyme reaction is conducted in a Kinase Buffer (50mM Tris pH 7.4, 20mM MgCl2, 0.1mg/ml BSA, 1mM DTT) containing 20uM ATP and 0.2mg/ml poly-(Glu-Tyr) for 30 min at 30°C. Enzyme activity is measured using Kinase-Glo plus reagents (Promega).
Application:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：10ug
Source Antigen：Recombinant, baculovirus
Grade：Purified
Purity：Purified (~51%)
Form：Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM sodium chloride, 2.4mM potassium chloride, 18mM glutathione, 10% glycerol.
