Human FGFR4, (GenBank Accession No. NM_002011) with N-terminal GST tag, MW= 65kD, expressed in Sf9 cells via a baculovirus expression system
Source:Recombinant protein corresponding to human FGFR4 at N-terminal, fused to GST tag expressed in Sf9 cells.
Molecular Weight:~65kD
Specific Activity:50pmole/min/ug
Assay Conditions:Reaction is conducted in a Kinase Buffer (50mM Tris pH 7.4, 20mM MgCl2, 0.1mg/ml BSA and 1mM DTT) containing 20uM ATP and 0.2mg/ml poly-(Glu-Tyr) for 30 min at 30°C. Enzyme activity is measured using Kinase-Glo plus reagents (Promega).
Application:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, baculovirus
Grade：Purified
Purity：~79% (SDS-PAGE)
Form：Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 4mM Glutathione, 3mM DTT, 20% glycerol.
