Recombinant full-length human CDK5 (59kD) and human p35 (60kD), both with N-terminal GST tags, were co-expressed in Sf9 cells using a baculovirus expression system. The CDK5 gene accession number is NM_004935 and p35 gene accession number is NM_003885.
Source:Full length recombinant protein corresponding to human CDK5 at N-terminal, fused to GST tag expressed in Sf9 cells using a baculovirus expression system.
Molecular Weight:~59kD, 49kD
Specific Activity:180pmole/min/ug
Assay conditions:Assay was done in Kinase buffer containing 0.2mM DTT using Histone 1 substrate (0.2mg/ml) and 20mM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred.
Application:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, baculovirus
Grade：Purified
Purity：~60%
Form：Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 16mM glutathione, 3mM DTT, 20% glycerol.
