Human Caspase 8 (CASP8), GenBank Accession No. NM_001228), aa200-496 (end), with C-terminal His-tag, MW=34.5kD, expressed in an E. coli cell expression system. Procaspase-8 is autocleaved between the prodomain and large protease subunit (p18). The cleavage product, p30, contains both the large (p18) and small (p10 [His-tag]) protease subunits. p30 is further processed to the p18 and p10 subunits, which dimerize to form active enzyme.
Source:Recombinant protein corresponding to aa200-496 from Caspase-8 at C-terminus, fused to His-tag expressed in E.coli.
Molecular Weight:~34.5kD
Specific Activity:1145pmol/min/ug
Application:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：50ug
Source Antigen：Recombinant, E.coli
Grade：Highly Purified
Purity：~99% (SDS-PAGE)
Form：Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 200mM imidazole, 3mM DTT, 20% glycerol.
