Human caspase 3 (CASP3), also known as CPP32 and apopain, aa1-277 (end) (GenBank Accession No. NM_004346, var alpha) with C-terminal His-tag, expressed in an E. coli expression system. Procaspase-3 is self-cleaved into two subunits, MW=17kD and 13kD, to form the active heterotetrameric complex
Source:Recombinant protein corresponding to aa1-277 from Caspase-3 at C-terminus, fused to His-tag expressed in E.coli.
Molecular Weight:~17kD and ~13kD, to form the active heterotetrameric complex.
Specific Activity:6784pmol/min/ug
Application:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Source Antigen：Recombinant, E.coli
Grade：Highly Purified
Purity：~99%
Form：Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 0.04% Tween 20, 3mM DTT, 20% glycerol.
