Caspase-3 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. The Homogeneous Caspase-3 BioAssay™ Kit is a complete assay system designed to measure Caspase-3 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent Caspase-3 activity measurements. In addition, the kit includes purified Caspase-3 enzyme and a potent Caspase-3 inhibitor, Ac-DNLD-CHO, for use as a positive and negative control. Using this kit, only one simple step on a microtiter plate is needed to analyze the Caspase-3 activity level. The fluorogenic substrate, Ac-DEVD-AFC, is incubated with purified Caspase-3 and the enzymatic activity releases AFC fluorophore that can then be measured using a fluorescence reader.
Applications:Great for studying enzyme kinetics and HTS applications.
Components:Recombinant Human Caspase-3, 1x5ugCaspase-3 Substrate (0.1mM), 1x50ul5X Caspase Assay Buffer 1, 1x20mlDTT (0.5M), 1x200ulAc-DNLD-CHO, 1x20ulBlack, low binding NUNC black microtiter plate, 1 plate
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
