Recombinant protein corresponding to aa2-321 of BirA, with a N-Terminal His-FLAG tag expressed in E. coli (NP_418404).
Molecular Weight:~37.1kD
Enzyme Activity:BirA (10ug/ml) completely biotinylated 10uM of substrate MBP-Avi at 30°C for 30 minutes in the standard reaction buffer containing 50mM Bicine pH 8.3, 10mM ATP, 10mM MgOAc, 50uM d-Biotin
Application:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：100ug
Source Antigen：E. coli
Grade：Purified
Purity：~65%
Form：Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 20% glycerol.
