Biotin ligase BirA, GenBank Accession No. NP_418404, a.a.2-321 with N-terminal GST-tag, MW = 61kD, expressed in an E. coli cell expression system.
Source:Recombinant protein corresponding to aa2-321 of BirA at the N-terminus, fused to GST tag expressed in E.coli.
Molecular Weight:~61kD
Application:Useful for covalently biotin-labeling of acceptor proteins for detection and purification assays.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size:100ug
Source Antigen:Recombinant, E. coli
Grade:Purified
Purity:~78%
Form:Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 16mM glutathione, 3mM DTT, 20% glycerol.