Human anaplastic lymphoma receptor tyrosine kinase (ALK), also known as CD246 and NBLST3, (GenBank Accession No. NM_004304), a.a. 1058-1620 (end) with N-terminal GST tag, MW = 89kD, expressed in a Baculovirus infected Sf9 cell expression system.
Source:Recombinant protein corresponding to aa1058-1620 from human ALK at N-terminal, fused to GST tag, expressed in baculovirus infected Sf9 cell expression system.
Molecular Weight:~89kD
Specific Activity:77 pmole/min/ug
Assay Conditions:Assay was done in a Kinase buffer using 0.2 mg/ml IGF1Rtide as a substrate with 20 uM ATP at 30oC for 40 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Application:Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, baculovirus
Grade：Purified
Purity：~60%
Form：Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 3mM DTT, 20% glycerol.
