Proteinase 3 is one of the serine proteinases localized in azurophil granules of polymorphonuclear leukocytes. It has been identified as the autoantigen of Wegener granulomatosis, a systemic vasculitis complicated by melomonocyte proliferation. It exhibits proteolytic activity towards a range of substrates. It is physiologically inhibited by alpha1-antitrypsin. The protein has been described as having antibiotic and growth-promoting properties and causing emphysema when administered by tracheal insufflation.
Source:Human Neutrophil
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:ELISA: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：200ug
Source Antigen：Human Neutrophil
Grade：Purified
Purity：~90% (SDS-PAGE)
Form：Supplied as a liquid in PBS, pH 8.0, 0.4M sodium chloride, 20mM Hepes-Sodium Hydroxide, 0.02% Lubrol, 0.1% Bromo-nitro-dioxane/Methylisothiazolone.
