Components in this kit are prepared with pure chemicals according to our proprietary technology. Pro QPCR SuperMix is a 2x concentration of premix reagent including Hotstart DNA polymerase and specially formulated real time buffer designed for real-time PCR with fluorescent probe-based detection format.
Real–time or quantitative PCR (QPCR) allows quantification of DNA, cDNA, or RNA templates. QPCR is based on the detection of a fluorescent reporter molecule that increases as PCR products accumulate with each cycle of amplification. The use of fluorescent probe technologies reduces the risk of sample contamination while maintaining convenience, speed and high throughput screening capabilities. Pro QPCR SuperMix can be used with fluorescent probe technologies to perform real-time PCR. The Pro QPCR SuperMix is a ready-to-use, 2x- concentrated master mix that contains all the reagents (except primers, probe and templates) needed for running quantitative, real-time DNA detection assays, in the fluorescent probe-based detection assays such as TaqMan® or molecular beacons. Thepassive reference dye ROX is included in the Pro QPCR SuperMix for many real-time QPCR platforms.
QPCR SuperMix contains United States Biological’s Taq DNA polymerase with hot start capability. hot-start Taq DNA polymerase improves PCR amplification reactions by decreasing nonspecific amplification and preventing primer-dimer formation. This enzyme is activated after an initial seven to ten minutes heating at 95°C. And the real-time PCR buffer is specially formulated to provide superior specificity and increase amplification efficiency. This SuperMix can amplify and detect a broad range of DNAs or cDNAs, including those GC- or AT-rich targets.
Kit Components:Pro QPCR SuperMixROX reference Dye
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
