Components in this kit are prepared with pure chemicals according to our proprietary technology. Fast Pro QPCR SuperMix-ROX premixed is a 2x concentration of qPCR reagent including Taq DNA polymerase and a specially formulated real time buffer designed for real-time PCR with fluorescent probe-based detection format.
Real–time or quantitative PCR (QPCR) allows quantification of DNA, cDNA, or RNA templates. QPCR is based on the detection of a fluorescent reporter molecule that increases as PCR products accumulate with each cycle of amplification. The use of fluorescent probe technologies reduces the risk of nonspecific products while maintaining convenience, speed and high throughput screening capabilities. The Fast Pro QPCR SuperMix is a ready-to-use, 2x concentrated master mix that contains all the reagents (except primers, probe and templates) needed for running quantitative real-time DNA detection assays utilizing fluorescent probe-based technologies such as TaqMan® or molecular beacons. The passive reference dye ROX is premixed in the Fast Pro QPCR SuperMix – ROX premixed.
QPCR SuperMix contains Taq DNA polymerase. The real-time PCR buffer is specially formulated to provide superior specificity and increase amplification efficiency. This superMix can amplify and detect a broad range of DNAs or cDNAs, including those GC- or AT-rich targets.
Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
