Granzyme A is a member of the granzyme family. Granzyme A is the most abundant serine protease in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A has a specific function in CTL and NK cells. It induces caspase-independent cell death when introduced into target cells by perforin. Human granzyme A is synthesized as a precursor (262 residues) with a signal peptide (residues 1-26), a propeptide (residues 27-28) and a mature chain (residues 29-262).
Recombinant protein corresponding to Glu27-Val262 from human Granzyme A with a C-terminal 10 His tag and a linker sequence in expressed in 293E cell line. Swiss/UniProt Accession: P12544
Function:Granzyme A is able to induce target cell apoptosis by activating caspase independent pathways.
Interaction:Targets of CTL and NK cells.
Molecular Weight:252 amino acid recombinant protein has a predicted molecular mass of approximately 28kD. The protein migrates at about 35kD in DTT-reducing conditions and about 55kD in non-reducing conditions by SDS-PAGE.The predicted N-terminal amino acid is Cys.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
