Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily.This heme protein catalyzes oxidations by hydrogen peroxide, including MPO- chloride mediated killing of microbes and tumor cells, inactivation of hemotactic factors, crosslinking of proteins and inclination of proteins.
Source:Human Neutrophil
Molecular Weight:~150kD
Biological Activity:180-220units/mg protein after lyophilization.
Unit Definition:The amount of enzyme that will decompose one micromole of hydrogen peroxide/minute at 25°C, pH 6.1. Reaction mixture contains 33mM sodium phosphate, pH 6.1, 30mM guaiacol, 0.0012% (0.35mM) hydrogen peroxide
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:100ug
Source Antigen:Human Neutrophil
Grade:Highly Purified
Purity:~95% (SDS-PAGE)
Form:Supplied as a lyophilized powder in 50mM Na acetate, pH 6.0, 100mM sodium chloride. Reconstitute in ddH2O.