Taenia solium is a tapeworm of 2-7m in length which resides in the small intestine of humans but also other animal species (monkeys, hamsters). The tape worms produce proglottids (less than 1000, and each with 50,000 eggs) which mature, become gravid, detach from the tapeworm, and migrate to the anus or are passed in the stool. The eggs contained in the gravid proglottids and passed with the feces can survive for months to years in the environment. After ingestion of a suitable intermediate host (pigs and other animals, including humans) the eggs release the oncosphere, invade the intestinal wall and migrate to the striated muscles, into the brain, liver and other tissues of the host where they develop in cysticerci. In the human intestine, a cysticercus develops over 2 months into an adult tapeworm, which can survive for up to 25 years. The important parasitic infection caused by Taenia solium is cysticer cosis which may involve the eye and the central nervous system. The swine tapeworm Taenia solium is worldwide in distribution. Prevalence is higher in poorer communities where humans live in close contact with pigs and eat undercooked pork, and is very rare in Muslim countries. The main symptom of Taeniasis (only mild) is often the passage (passive) of proglottids. The most important feature of Taeniasis solium is the risk of development of Cysticercosis.
Intended Use:The Taenia solium IgG ELISA is intended for the qualitative determination of IgG class antibodies against Taenia solium in human serum or plasma (citrate).
Test Principle:The qualitative immunoenzymatic determination of IgG-class antibodies against Taenia solium is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with Taenia solium antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled Protein A conjugate is added. This conjugate binds to antigen- antibody complexes. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Taenia solium-specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint color. Absorbance at 450nm is read using an ELISA microwell plate reader
Kit Components:C9046A: Taenia solium Coated Wells (IgG): 1x96wells 12 break apart 8-well snap-off strips coated with Taenia solium antigens; in resealable aluminium foil. C9046B: IgG Sample Diluent, 1x100ml of buffer for sample dilution; pH 7.2 ± 0.2; Colored yellow; ready to use; white cap. Contains 0.1 % KathonC9046C: Stop Solution, 0.2M H2SO4 1x15ml, ready to use; red cap. C9046D: Washing Solution, 20X 1x50ml 20-fold concentrated buffer (pH 7.2 ± 0.2) for washing the wells; white cap. Contains 0.1 % Bronidox L after dilutionC9046E: Protein A (HRP) 1x20ml of peroxidase-conjugated Protein A. Colored blue, ready to use; black cap. Contains 0.2 % Bronidox LC9046F: TMB Substrate Solution, 1x15ml 3,3',5,5'-tetramethylbenzidine (TMB); ready to use; yellow cap. C9046G: Taenia solium IgG Positive Control, 1x 2ml; Colored yellow; ready to use; red cap. Contains 0.1 % KathonC9046H: Taenia solium IgG Cut-off Control, 1x 3ml; Colored yellow; ready to use; green cap. Contains 0.1 % KathonC9046J: Taenia solium IgG Negative Control, 1x2ml; Colored yellow; ready to use; blue cap. Contains 0.1 % Kathon.
Storage and Stability:Store all kit components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
