Heparanase is an endobeta-D-glucuronidase, which degrades heparansulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes (1,2,3). Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis (4,5). Heparanase is a heterodimer comprised of a 50kD subunit harboring the active site and a 8kD subunit. It is produced as a latent 65kD precursor and proteolytically processed to its active form (1,6). Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in somecases with increased tumor invasiveness and vascularity and with poor prospective survival (7,8).
Applications:Suitable for use as positive control in Western Blot. Other applications not tested.
Recommended Dilution:Western Blot: Use 20ul per lane, as a control for using H1890-95 (Heparanase 1 antibody)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ng
Source Antigen：Recombinant, CHO cells
Grade：Purified
Purity：Purified by several orthogonal chromatography steps.
Form：Supplied as a liquid in LDS-PAGE buffer (140 mM Tris buffer pH 8.5, 10% Glycerol, 2% LDS, 0.015% EDTA, 1.88% (v/v) of 1% Serva Blue G250 and 0.625% (v/v) of 1% Phenol red)
