Transferrin receptors are transmembrane, homodimeric proteins that play a role in iron transport, uptake, and storage. TfR is an essential receptor, found on almost all cell types, regulated by intracellular iron concentration, and has a high affiinity for transferrin. Soluble TfR (sTfR) arises from the proteolysis of TfR into monomers that can be measured in plasma and serum. TfR2 has limited tissue distribution (hepatocytes and enterocytes), is regulated by the cell cycle, and binds transferrin poorly.
Intended Use:TfR BioAssay™ ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant Human TfR (Transferrin R). The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use.
Each kit contains sufficient materials to run ELISAs on approximately 15x96-well plates, provided that the following conditions are met: The assay is run as summarized in the General ELISA protocol. The recommended microplates, buffers, diluents, substrates and solutions are used.
Storage and Stability:Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
