Phosphatase Receptor-type sigma (PTPRs), also known as PTPs, is a 200kD type I transmembrane glycoprotein that is a member of the class 2A (LAR) subfamily of the protein tyrosine phosphatase (PTP) family (1). Human PTPRs cDNA encodes 1948aa including a 29aa signal sequence, a 1253aa extracellular domain (ECD) with three C2-type Ig domains and eight Fibronectin (Fn) type III domains, a transmembrane domain, and a cytoplasmic region with two PTP domains (1, 2). Multiple differentially expressed splice variants lack sections of the Ig and/or Fn domains and/or cytoplasmic juxtamembrane sequence (1, 2). PKC activation in the cell promotes endoprotease cleavage of PTPRs, releasing the 140-160kD ECD (3). The human PTPRs ECD shares 93%aa sequence identity with the nearest isoform of mouse and rat PTPRs, 96% with bovine PTPRs, and 97% with porcine PTPRs. Activity of PTPRs can be inhibited by bisphosphonates (2). PTPRs is a receptor for several proteins, including neural chondroitin sulfate proteoglycans which inhibit nerve regeneration (4, 5). It also binds and dephosphorylates targets such as N-Cadherin, E-Cadherin, EGF R and Ret (6-9). Through these interactions PTPRs affects pituitary and neuroendocrine development, and axon growth and targeting (4-6). Mice deleted for PTPRs show pituitary, pancreatic, and gut abnormalities, mainly due to growth hormone insufficiency (9-12). They also show enhanced regeneration, but faulty targeting, of injured peripheral nerves (4-6, 13). Head and neck squamous cell cancers frequently underexpress PTPRs (14). Since PTPRs inhibits EGF R signaling pathways, its underexpression can enhance tumor cell growth (14).
Source:Recombinant corresponding to aa30-1260 from human PTPRs at C-terminal, fused to 6-His tag expressed in NS0 cells (NP_570924).
Molecular Weight:~135.8kD
Biological Activity:Measured by its binding ability in a functional ELISA. When Recombinant Human PTPR sigma is coated at 2ug/ml, rhTrkC Fc Chimera binds with KD <1nm.
Endotoxin:<0.10 EU per 1ug of protein by the LAL method
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, NS0 cells
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a lyophilized powder in HEPES, NaCl. Reconstitute with sterile PBS to 500ug/ml.
