Based on its helical structure, LIF (Leukemia Inhibitory Factor) is considered a member of the Interleukin-6 family of cytokines. Functionally, it has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. Some cell types known to express LIF include activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts.
Recombinant protein corresponding to Pro24-Phe202 from human LIF, expressed in E. coli.
Molecular Weight:~19.6kD
Biological Activity:Measured by its ability to induce IL-6 secretion by M1 mouse myeloid leukemia cells. The ED50 for this effect is ~0.5ng/ml.
Endotoxin:<0.1EU/1ug (LAL)
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥95% (SDS-PAGE)
Form：Supplied as a lyophilized powder in PBS. No BSA added. Reconstitute with 250ul sterile PBS.
