Source:Recombinant protein corresponding to Human GMP, Pro143-Ser288, with an N-terminal Ala, expressed in E. coli.
Molecular Weight:~16.5kD
Biological Activity:Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. The ED50 for this effect is 0.1-0.6ng/ml.
Specific Activity:~800 IU/ug, which is calibrated against human FGF basic WHO International Standard (NIBSC code: 90/712).
Endotoxin:<0.10EU/1ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Produced using non-animal reagents in an animal-free laboratory.
仕様
Size：25ug
Source Antigen：Recombinant, Human GMP, at N-terminal Ala expressed in E. coli.
Grade：Highly Purified
Purity：~97% (SDS-PAGE)
Form：Supplied as a lyophilized powder in Tris-HCl, NaCl. Reconstitute with 250ul sterile PBS.
