Tris-NTA, can be used as a chelating agent for fluorescence labeling of histidine-tagged proteins to lipid membranes, which is a powerful mean for studying and monitoring protein interaction in cells.
Synonyms:(α1S,α4S,α8S)-11-(6-Amino-1-oxohexyl)-α1,α4,α8-tris[bis(carboxymethyl)amino]-δ1,δ4,δ8-trioxo-1,4,8,11-tetraazacyclotetradecane-1,4,8-tripentanoic Acid Trifluoroacetic Acid Salt; (αS,α’S,α’’S)-11-(6-Amino-1-oxohexyl)-α,α’,α’’-tris[bis(carboxymethyl)amino]-δ,δ’,δ’’-trioxo-1,4,8,11-tetraazacyclotetradecane-1,4,8-tripentanoic Acid Trifluoroacetic Acid Salt
Molecular Formula:C45H69F3N8O24
Molecular Weight:1163.06
Purity:≥94%
Appearance:White to off-white solid
Solubility:Methanol (Slightly), Water (Slightly)
Melting Point:>170°C (dec.) (lit)
Atmosphere:Inert
Method for Determining Identity:Proton NMR Spectroscopic and Mass Spectrometric Analysis
TLC Conditions:C18; Methanol: Water=9:1; Visualized with Ninhydrin; Rf=0.25
Storage and Stability:May be stored at RT for short-term only. Long-term storage is recommended at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Hygroscopic. Store under inert atmosphere.
