High affinity RAD51 activator (Kd range = 48-107nM, value depends on cofactors present). Enhances homologous recombination activity of hRAD51. Enhances homology-directed repair and increases CRISPR-mediated knock-in efficiencies in vitro and in vivo.
RS-1 is an activator of DNA repair protein RAD51 (Kd = 48-107 for human RAD51 with different cofactors present).1 It stimulates homologous strand assimilation activity at least 5- to 11-old, enhancing homologous recombination activity of hRAD51.1 RS-1 is a potent enhancer of CRISPR-mediated genome editing, increasing homology directed repair 3- to 6-fold.2 It is used to increase CRISPR-mediated knock-in efficiencies in vitro and in vivo.3,2,4
Solubility:RS-1 is supplied as a crystalline solid. A stock solution may be made by dissolving the RS-1 in the solvent of choice. RS-1 is soluble in organic solvents such as DMSO and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of RS-1 in these solvents is approximately 20 and 30 mg/ml, respectively
RS-1 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, RS-1 should first be dissolved in DMF and then diluted with the aqueous buffer of choice. RS-1 has a solubility of approximately 0.2 mg/ml in a 1:4 solution of DMF:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Storage and Stability:Store at room temperature. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
