Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several death receptors including DR3, DR4, DR5, and DR6 have been identified. Like TNF-R1, DR6 (also known as TNFRSF21) interacts with death domain containing adapter molecule TRADD. Overexpression of DR6 induces apoptosis and activates NF-kB and JNK. DR6 is widely expressed in human tissues and cell lines. Recently, DR6 was found to interact with an amino-terminal fragment of the Beta-amyloid protein (APP) in neurons, activating a caspase 6-dependent apoptotic event leading to axonal degeneration and pruning. The doublet observed in the SDS-PAGE is likely due to incomplete removal of the signal sequence.
Source:Recombinant protein corresponding to human DR6 Cytoplasmic Domain, fused to His-tag at C-terminal expressed in E.coli.
Molecular Weight:~36kD
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~95%
Form：Supplied as a liquid in 1X PBS, 10% glycerol.
