IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6Rα and gp130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant human IL-6 is a 20.9kD protein containing 184aa. Manufactured using all non-animal reagents.
Source:Recombinant protein corresponding to human IL-6, expressed in E.coli.
Biological Activity:Determined by the dose-dependent stimulation of the proliferation of the IL-6 dependent murine 7TD1 cells. The ED50 was found to be 0.1ng/ml
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥98% by SDS-PAGE and HPLC. Endotoxin: ≤0.1ng/ug.
Form：Reconstitute with sterile ddH2O to a concentration of 0.1-0.5mg/ml. Gently shake the solution for about 10 minutes for better solubility. Do not vortex.
