85-1960-63　Nerve Growth Factor, beta, Recombinant, Human (Beta-nerve Growth Factor, Beta-NGF, NGFb, NGF, HSAN5, MGC161426, MGC161428) 20ug　149732

特徴

• Beta-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Beta-NGF is a potent neurotrophic factor that signals through its receptor beta-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Beta-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. The functional form of human beta-NGF is a noncovalently disulfide-linked homodimer, of two 13.5kD polypeptide monomers (238aa). The three disulfide bonds are required for biological activity.
• Source:Recombinant protein corresponding to human NGF beta, expressed in E.coli.
• Biological Activity:Determined by its ability to stimulate chick E9 DRG neurite outgrowth. The ED50 is <1ng/ml.
• Specific Activity:>1x10e6 units/mg
• Endotoxin:<0.1ng/ug (1EU/ug)
• AA Sequence:SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVR
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.