85-1960-45　Fibroblast Growth Factor, Basic, Recombinant, Rat (FGF2, BFGF, FGFB, HBGF-2, HBGH-2, Heparin-binding Growth Factor 2 Precursor, Prostatropin) 50ug　149723

特徴

• FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant rat FGF-basic is a 16.3kD protein consisting of 145aa.
• Source:Recombinant protein corresponding to rat FGF-basic, expressed in E.coli.
• Biological Activity:Determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells. The ED50 was found to be 0.2ng/ml
• Specific Activity:5x10e6units/mg
• Endotoxin:<0.1ng/ug (1EU/ug)
• AA Sequence:PALPEDGGGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.