85-1960-35　Tnfsf10, Recombinant, Mouse (Tumor Necrosis Factor Ligand Superfamily Member 10, TNF-related Apoptosis-inducing Ligand, Protein TRAIL, CD253, Trail, A330042I21Rik, AI448571) 50ug　149712

特徴

• TRAIL is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic “death domain”, which activates the cell's apoptotic machinery. Recombinant murine soluble TRAIL is a 174aa polypeptide, 20kD, consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL protein.
• Source:Recombinant protein corresponding to mouse TRAIL, expressed in E.coli.
• Biological Activity:Determined by the dose-dependent stimulation of MIP-2 production by mouse spleen cells using a concentration range of 10-100ng/ml.
• Endotoxin:<0.1ng/ug (1EU/ug)
• AA Sequence:MRGGRPQKVA AHITGITRRS NSALIPISKD GKTLGQKIES WESSRKGHSF LNHVLFRNGE LVIEQEGLYY IYSQTYFRFQ EAEDASKMVS KDKVRTKQLV QYIYKYTSYP DPIVLMKSAR NSCWSRDAEY GLYSIYQGGL FELKKNDRIF VSVTNEHLMD LDQEASFFGA FLIN
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.