Growth Differentiation Factor 11 (GDF11) BioAssay™ ECL Kit (Rat) is a quantitative, in vitro, Chemiluminescence-based sandwich immunoassay (ECL) suitable for use in rat serum, plasma, tissue homogenates and other biological fluids.
Test Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 11. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Differentiation Factor 11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Growth Differentiation Factor 11 (GDF11) level in the sample or standard.
Detection Range:1.37-1000pg/ml
Sensitivity:0.53pg/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*157682A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use*157682B: Standard, 2x1 vial157682C: Standard Diluent, 1x20ml*157682D: Detection Reagent A (green), 1x120ul*157682E: Detection Reagent B (red), 1x120ul157682F: Assay Diluent A, 1x12ml157682G: Assay Diluent B, 1x12ml157682H: Substrate A, 1x10ml157682K: Substrate B, 1x10ml157682L: Wash Buffer, 30X, 1x20ml
Precaution:The Stop Solution (157682K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *157682A, *157682B, *157682D and *157682E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary:1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C
3. Add 100ul prepared Detection Reagent A immediately. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 5. Aspirate and wash 5 times
6. Add 100ul Substrate Solution. Incubate 10 minutes at 37°C. 7. Read RLU value immediately.
