Intended Use:Fibroblast Growth Factor Binding Protein 3 (FGFBP3) BioAssay™ ECL Kit (Human) is an Electrochemical luminescence-based immunoassay (ECL) designed for the in vitro sensitive quantitative measurement of FGFBP3 in human tissue homogenates, cell lysates and other biological fluids.
Detection Range:27.4-20,000pg/ml
Sensitivity:11.3pg/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*157644A: Microtiter Plate, 1x96 wells*157644B: Standard, 2x1 vial157644C: Standard Diluent, 1x20ml*157644D: Detection Reagent A (green), 1x120ul*157644E: Detection Reagent B (red), 1x120ul157644F: Assay Diluent A, 1x12ml157644G: Assay Diluent B, 1x12ml157644H: Substrate A, 1x10ml157644K: Substrate B, 1x2ml157644L: Wash Buffer (30X), 1x20ml
Storage and Stability:Store *157644A, *157644B, *157644D and *157644E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 100ul Substrate Solution. Incubate 10 minutes at 37°C
