The Fibroblast Growth Factor 18 (FGF18) BioAssay™ ECL Kit is a sensitive chemiluminescent immunoassay for the in vitro quantitative measurement of FGF18 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range:1.37-1000pg/ml
Sensitivity:0.55pg/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*157613A: Microtiter Plate, 1x96 wells, Pre-coated with antibody; ready to use
*157613B: Standard, 2x1vial 157613C: Standard Diluent, 1x20ml*157613D: Detection Reagent A, 1x120ul *157613E: Detection Reagent B, 1x120ul 157613F: Assay Diluent A, 1x12ml157613G: Assay Diluent B, 1x12ml157613H: Substrate A, 1x10ml*157613J: Positive Control, 1x1vial, 87.1pg/ml (Lyophilized)157613K: Substrate B, 1x2ml157613L: Wash Buffer, 30X, 1x20ml
Storage and Stability:Store *157613A, *157613B, *157613D, *157613E and *157613J at -20°C. After reconstitution of *157613J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Notes on 157613J: Positive Control:Intended Use:The positive control is from a human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
Characteristics:The control vials contain human FGF18 in human serum (lyophilized powder). Note: The human based serum used in this product are screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.
Expected Values:87.1pg/ml
Usage:Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
