85-1913-89　Thrombospondin 1 (THBS1) Recombinant, Rat 200ug　156989

特徴

• Recombinant protein corresponding to Asn327-Ala478 of rat Thrombospondin 1 with a N-terminal His-tag expressed in E. coli. (Q71SA3)
• Purity:≥95%
• Amino Acid Sequence:NNEE WTVDSCTECH CQNSVTICKK VSCPIMPCSN ATVPDGECCP RCWPSDSADD GWSPWSEWTS CSATCGNGIQ QRGRSCDSLN NRCEGSSVQT RTCHIQECDK RFKQDGGWSH WSPWSSCSVT CGDGVITRIR LCNSPSPQMN GKPCEGEA
• Predicted isoelectric point:5.8
• Predicted Molecular Weight:18.2kD
• Accurate Molecular Mass:20kD as determined by SDS-PAGE reducing conditions
• Subcellular Location:Secreted, cytoplasm
• Applications:Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C..Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note:Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.