85-1902-20　Receptor Interacting Serine Threonine Kinase 3 (RIPK3) Recombinant, Mouse 200ug　156589

特徴

• Recombinant protein corresponding to aa16-239 with two N-terminal Tags, His-Tag and T7-Tag from human RIPK3 expressed in E. coli (Q9QZL0)
• Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LVSRE ELKKLEFVGK GGFGVVFRAH HRTWNHDVAV KIVNSKKISW EVKAMVNLRN ENVLLLLGVT EDLQWDFVSG QALVTRFMEN GSLAGLLQPE CPRPWPLLCR LLQEVVLGMC YLHSLDPPLL HRDLKPSNIL LDPELHAKLA DFGLSTFQGG SQSGSGSGSG SRDSGGTLAY LDPELLFKVN LKASKASDVY SFGILVWAVL AGREAELVDK TSLIRETVC
• Predicted Molecular Weight:28.5kD
• Predicted Isoelectric Point:7.2
• Subcellular Location:Cytoplasm, Cell Membrane, Mitochondrion
• Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Included Protein Molecular Weight Marker:168631: Unstained Protein Molecular Weight Marker, 10-70kD. No heating, diluting or reducing agents needed
• MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kDSupplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt.
• Storage and Stability:Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Note:Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.