85-1892-28　Perforin 1 (PRF1) Recombinant, Mouse 10ug　156257

特徴

• Recombinant protein corresponding to aa40-355 with an N-terminal His and GST tag from mouse Perforin 1, produced in E. coli. Swiss/Uniprot Number: P10820.
• Amino Acid Sequence:V WMAGEGMDVT TLRRSGSFPV NTQRFLRPDR TCTLCKNSLM RDATQRLPVA ITHWRPHSSH CQRNVAAAKV HSTEGVAREA AANINNDWRV GLDVNPRPEA NMRASVAGSH SKVANFAAEK TYQDQYNFNS DTVECRMYSF RLVQKPPLHL DFKKALRALP RNFNSSTEHA YHRLISSYGT HFITAVDLGG RISVLTALRT CQLTLNGLTA DEVGDCLNVE AQVSIGAQAS VSSEYKACEE KKKQHKMATS FHQTYRERHV EVLGGPLDST HDLLFGNQAT PEQFSTWTAS LPSNPGLVDY SLEPLHTLLE EQNPK
• Predicted Molecular Mass:67.2kD
• Predicted Isoelectric Point:7.4
• Accurate Molecular Mass:65kD as determined by SDS-PAGE reducing conditions
• Subcellular Localization:Membrane, Secrested, Cytoplasm. Endosome
• Applications:Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Note:Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.