85-1869-55　Kallikrein 1 (KLK1) Recombinant, Human 50ug　155488

特徴

• Recombinant protein corresponding to Ile25~Asn261 with an N-Terminal His-tag from human expressed in E. coli Swiss/Uniprot Accession: P06870
• AA Sequence:MGHHHHHHSGSEF-IVGGWE CEQHSQPWQA ALYHFSTFQC GGILVHRQWV LTAAHCISDN YQLWLGRHNL FDDENTAQFV HVSESFPHPG FNMSLLENHT RQADEDYSHD LMLLRLTEPA DTITDAVKVV ELPTEEPEVG STCLASGWGS IEPENFSFPD DLQCVDLKIL PNDECKKAHV QKVTDFMLCV GHLEGGKDTC VGDSGGPLMC DGVLQGVTSW GYVPCGTPNK PSVAVRVLSY VKWIEDTIAE N
• Predicted Isoelectric Point:4.9
• Predicted Molecular Weight:27.8kD
• Accurate Molecular Mass:31kD as determined by SDS-PAGE under reducing conditions
• Note: The possible reasons that the actual band size differs from the predicted are as follows:1. Splice variants: Alternative splicing may create different sized proteins from the same gene2. Relative charge: The composition of amino acids may affect the charge of the protein
• 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc
• 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins and then cleaved to give the active form
• 5. Polymerization of the target protein: Dimerization, multimerization etc.
• Applications:Suitable for use in ELISA, Western Blot and SDS-PAGE
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store lyophilized powder at -70°C. Stable for 6 months after receipt.
• Note:Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.