85-1864-89　Interleukin 1 Receptor Associated Kinase 4 (IRAK4) Recombinant, Human 10ug　155331

特徴

• Recombinant protein corresponding to Gly189-Glu427 with an N-Terminal His-Tag of human IRAK4, expressed in E. coli (Q9NWZ3)
• Amino Acid Sequence:GN KMGEGGFGVV YKGYVNNTTV AVKKLAAMVD ITTEELKQQF DQEIKVMAKC QHENLVELLG FSSDGDDLCL VYVYMPNGSL LDRLSCLDGT PPLSWHMRCK IAQGAANGIN FLHENHHIHR DIKSANILLD EAFTAKISDF GLARASEKFA QTVMTSRIVG TTAYMAPEAL RGEITPKSDI YSFGVVLLEI ITGLPAVDEH REPQLLLDIK EEIEDEEKTI EDYIDKKMND ADSTSVE
• Predicted Isoelectric Point:5.1
• Predicted Molecular Weight:27.7kD
• Accurate Molecular Mass:31kD as determined by SDS-PAGE reducing conditions
• Note: The possible reasons that the actual band size differs from the predicted are as follows:1. Splice variants: Alternative splicing may create different sized proteins from the same gene2. Relative charge: The composition of amino acids may affect the charge of the protein
• 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc
• 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins and then cleaved to give the active form
• 5. Polymerization of the target protein: Dimerization, multimerization etc.
• Subcellular Location:Cytoplasm
• Applications:Suitable for use in ELISA, Western Blot and SDS-PAGE
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Note:Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.