85-1861-05　Insulin Like Growth Factor Binding Protein 5 (IGFBP5) Recombinant, Rat 200ug　155202

特徴

• Recombinant protein corresponding to His25-Glu271 of rat IGFBP5 with a N-terminal His-Tag expressed in E. coli. (P24594)
• Amino Acid Sequence:HCEPCD EKALSMCPPS PLGCELVKEP GCGCCMTCAL AEGQSCGVYT ERCAQGLRCL PRQDEEKPLH ALLHGRGVCL NEKSYGEQTK IERDSREHEE PTTSEMAEET YSPKVFRPKH TRISELKAEA VKKDRRKKLT QSKFVGGAEN TAHPRVIPAP EMRQESDQGP CRRHMEASLQ EFKASPRMVP RAVYLPNCDR KGFYKRKQCK PSRGRKRGIC WCVDKYGMKL PGMEYVDGDF QCHAFDSSNV E
• Predicted Isoelectric Point:8.5
• Predicted Molecular Weight:29.2kD
• Subcellular Location:Secreted
• Accurate Molecular Mass:35kD as determined by SDS-PAGE reducing conditions
• Note: The possible reasons that the actual band size differs from the predicted are as follows:1. Splice variants: Alternative splicing may create different sized proteins from the same gene2. Relative charge: The composition of amino acids may affect the charge of the protein
• 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc
• 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins and then cleaved to give the active form
• 5. Polymerization of the target protein: Dimerization, multimerization etc.
• Applications:Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.