85-1860-06　Inhibin Beta E (INHbE) Recombinant, Human 200ug　155168

特徴

• INHBE is a member of the activin beta family that plays a role in pancreatic exocrine cell growth and proliferation. Hashimoto et al. cloned INHBE, which they called activin beta-E subunit. The deduced 350aa protein contains an N-terminal hydrophobic signal peptide and a potential N-glycosylation site. The proposed cleavage site at aa232-236 would yield a mature 114aa protein. INHBE shares 97% and 96% similarity with the mouse and rat activin beta-E orthologs, respectively. The deduced mature human beta-E subunit shares aa identity of 44%, 48%, and 64% with activin beta-A, activin beta-B, and activin beta-C, respectively.
• Recombinant protein correspondign to Thr237-Ser350 with an N-terminal His-Tag from human INHbE, expressed in E. coli (P58166)
• Amino Acid Sequence:TPTC EPATPLCCRR DHYVDFQELG WRDWILQPEG YQLNYCSGQC PPHLAGSPGI AASFHSAVFS LLKANNPWPA STSCCVPTAR RPLSLLYLDH NGNVVKTDVP DMVVEACGCS
• Predicted Molecular Weight:13.7kD
• Predicted Isoelectric Point:6.2
• Tissue Specificity:Liver, Muscle
• Accurate Molecular Mass:17kD and 18kD as determined by SDS-PAGE reducing conditionsNote: The possible reasons that the actual band size differs from the predicted are as follows:1. Splice variants: Alternative splicing may create different sized proteins from the same gene2. Relative charge: The composition of amino acids may affect the charge of the protein
• 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc
• 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins and then cleaved to give the active form
• 5. Polymerization of the target protein: Dimerization, multimerization etc.
• Applications:Suitable for use in Western Blot and SDS-PAGE. Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Included Protein Molecular Weight Marker:168631: Unstained Protein Molecular Weight Marker, 10-70kDSuppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Ready to use; no need to heat, dilute or add reducing agents before use.
• Protein Bands: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD
• Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier.
• Usage: Allow the marker to reach room temperature and mix thoroughly before use to ensure that any solids that may have precipitated at -20°C will go back into solution. Do not boil! Load the following volumes on SDS-PAGE gel:Mini gels: 3-5ul/wellLarge gel: 7ul/wellThe loading volumes listed above are recommended for gels with thickness of 0.75-1mm. The loading volume should be doubled for 1.5mm thick gels
• Store at -20°C Stable for 6 months after receipt.
• Storage and Stability:Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Note:Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.