85-1844-33　Fibulin 1 (FBLN1) Recombinant, Human 10ug　154613

特徴

• Recombinant protein corresponding to aa176-398 with an N-terminal His-Tag from human FBLN1, expressed in E. coli (P23142)
• Amino Acid Sequence:MGHHHHHHSGSEF-LNDRC RGGGPCKQQC RDTGDEVVCS CFVGYQLLSD GVSCEDVNEC ITGSHSCRLG ESCINTVGSF RCQRDSSCGT GYELTEDNSC KDIDECESGI HNCLPDFICQ NTLGSFRCRP KLQCKSGFIQ DALGNCIDIN ECLSISAPCP IGHTCINTEG SYTCQKNVPN CGRGYHLNEE GTRCVDVDEC APPAEPCGKG HRCVNSPGSF RCECKTGYYF DGISRMCV
• Predicted Molecular Weight:25.7kD
• Predicted Isoelectric Point:5.4
• Subcellular Location:Secreted, extracellular space, extracellular matrix
• Accurate Molecular Mass:30kD as determined by SDS-PAGE reducing conditionsNote: The possible reasons that the actual band size differs from the predicted are as follows:1. Splice variants: Alternative splicing may create different sized proteins from the same gene2. Relative charge: The composition of amino acids may affect the charge of the protein
• 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc
• 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins and then cleaved to give the active form
• 5. Polymerization of the target protein: Dimerization, multimerization etc.
• Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Included Protein Molecular Weight Marker:168631: Unstained Protein Molecular Weight Marker, 10-70kD. No heating, diluting or reducing agents needed
• MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kDSupplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt.
• Storage and Stability:Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Note:Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.