85-1844-10　Fibronectin (FN) Recombinant, Human 50ug　154605

特徴

• Recombinant protein corresponding to aa2206-2337 (P02751) with a N-Terminal His-Tag from human Fibronectin expressed in E. coli
• Predicted Molecular Weight:16.1kD
• Predicted Isoelectric Point:5.3
• Subcellular Location:Secreted, extracellular space, extracellular matrix
• Amino Acid Sequence:ALSQTT ISWAPFQDTS EYIISCHPVG TDEEPLQFRV PGTSTSATLT GLTRGATYNV IVEALKDQQR HKVREEVVTV GNSVNEGLNQ PTDDSCFDPY TVSHYAVGDE WERMSESGFK LLCQCLGFGS GHFRCD
• Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Included Protein Molecular Weight Marker:168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed
• MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kDSupplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note:Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.