85-1835-65　Deiodinase, Iodothyronine, Type II (DIO2) Recombinant, Rat 10ug　154324

特徴

• Recombinant protein corresponding to Ser30-Phe259 of rat Deiodinase, Iodothyronine, Type II (DIO2) with a N-terminal His tag and MBP-tag expressed in E. coli. Swiss/UniProt Accession: P70551
• Amino Acid Sequence:S VILLKHVALL LSRSKSTRGE WRRMLTSEGL RCVWNSFLLD AYKQVKLGED APNSSVVHVS NPEAGNNCAS EKTADGAECH LLDFASAERP LVVNFGSATU PPFTRQLPAF RQLVEEFSSV ADFLLVYIDE AHPSDGWAVP GDSSMSFEVK KHRNQEDRCA AAHQLLERFS LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG KGPFSYNLQE VRSWLEKNF
• Molecular Weight:72.4kD
• Accurate Molecular Mass:60kD as determined by SDS-PAGE reducing conditions
• Phenomenon Explanation:The possible reasons that the actual band size differs from the predicted are as follows:1.Splice variants: Alternative splicing may create different sized proteins from the same gene
• 2. Relative charge: The composition of amino acids may affects the charge of the protein
• 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc
• 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form
• 5. Polymerization of the target protein: Dimerization, multimerization etc.
• Predicted Isoelectric Point:5.8
• Subcellular Localization:Membrane
• Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Note:Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.