85-1832-03　Corticotropin Releasing Hormone (CRH) Recombinant, Rat 50ug　154201

特徴

• Product Description:E. coli-expressed recombinant protein mapping to residues encompassed by Gnl39 thru Lys187 of rat Corticotropin Releasing Hormone (CRH), UniProt Accession: P01143. Fused to N-terminal His tag.
• Amino Acid Sequence:MGHHHHHHSGSEF-QP LNFLQPEQPQ QPQPILIRMG EEYFLRLGNL NRSPAARLSP NSTPLTAGRG SRPSHDQAAA NFFRVLLQQL QMPQRPLDSS TELAERGAED ALGGHQGALE RERRSEEPPI SLDLTFHLLR EVLEMARAEQ LAQQAHSNRK LMEIIGK
• Molecular Weight:Predicted: 18.3kDObserved: 19kDa (by reducing SDS-PAGE)
• Predicted Isolectric Point:6.6
• Subcellular Localization:Secreted
• Applications:Suitable for use as positive control and as antigen in immunogenic applications. May also be used in Western Blot. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -70°C and is stable for 6 months after receipt. Upon reconstitution, aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Note:Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.