85-1827-67　Coagulation Factor IX (F9) Recombinant, Bovine 200ug　154053

特徴

• Recombinant protein corresponding Leu319-Thr462 with an N-terminal His-Tag of bovine Coagulation Factor IX, expressed in E. coli (P00741)
• Amino Acid Sequence:LLELDEPL ELNSYVTPIC IADRDYTNIF SKFGYGYVSG WGKVFNRGRS ASILQYLKVP LVDRATCLRS TKFSIYSHMF CAGYHEGGKD SCQGDSGGPH VTEVEGTSFL TGIISWGEEC AMKGKYGIYT KVSRYVNWIK EKTKLT
• Predicted Isoelectric Point:8.2
• Predicted Molecular Weight:17.7kD
• Accurate Molecular Mass:20kD as determined by SDS-PAGE reducing conditions
• Subcellular Location:Secreted
• Applications:Suitable for use in ELISA, Western Blot nd SDS-PAGE. Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Note:Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.