85-1813-13　Apolipoprotein A1 (APOA1) Recombinant, Mouse 10ug　153557

特徴

• Recombinant protein corresponding to Asp25-Gln264 with N-Terminal His Tag from mouse APOA1, expressed in E. coli Swiss/UniProt Accession: Q00623.
• Amino Acid Sequence:DEPQSQ WDKVKDFANV YVDAVKDSGR DYVSQFESSS LGQQLNLNLL ENWDTLGSTV SQLQERLGPL TRDFWDNLEK ETDWVRQEMN KDLEEVKQKV QPYLDEFQKK WKEDVELYRQ KVAPLGAELQ ESARQKLQEL QGRLSPVAEE FRDRMRTHVD SLRTQLAPHS EQMRESLAQR LAELKSNPTL NEYHTRAKTH LKTLGEKARP ALEDLRHSLM PMLETLKTKA QSVIDKASET LTAQ
• Predicted Isoelectric Point:5.3
• Predicted Molecular Weight:31.6kD
• Accurate Molecular Weight:28kD as determined by SDS-PAGE reducing conditions
• Subcellular Location:Secreted
• Applications:Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Note:Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.