85-1808-97　Alanine Aminotransferase (ALT) Recombinant, Mouse 200ug　153413

特徴

• Recombinant protein corresponding to Arg-Gln417 with an N-Terminal His-Tag from mouse ALO, expressed in E. coli.
• Predicted Isoelectric Point:5.6
• Predicted Molecular Weight:34kD
• Accurate Molecular Mass:33kD as determined by SDS-PAGE reducing conditions
• Subcellular Location:Cytoplasm
• Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Note:Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.