The Semaphorin 3C (SEMA3C) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SEMA3C in human serum, plasma, tissue homogenates and other biological fluids.
Detection Range:78-5000pg/ml
Sensitivity:30pg/ml
Intra-Assay:CV<10%
Inter-Assay:CV<12%
Kit Components:*153100A: Microtiter Strips, 1x96wells, Pre-coated, ready to use*153100B: Standard, 2x1vial 153100C: Standard Diluent, 1x20ml*153100D: Detection Reagent A (green), 1x120ul *153100E: Detection Reagent B (red), 1x120ul 153100F: Assay Diluent A, 1x12ml153100G: Assay Diluent B, 1x12ml153100H: TMB Substrate, 1x9ml*153100J: Positive Control, 1820pg/ml, 1x1vial (Lyophilized)153100K: Stop Solution, 1x6ml153100L: Wash Buffer, 30X, 1x20ml
Precaution:The Stop Solution (153100K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *153100A, *153100B, *153100D, *153100E and *153100J at -20°C. After reconstitution of *153100J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
