This BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PRM1 in human tissue homogenates, cell lysates and other biological fluids
Detection Range:0.156-10ng/ml
Sensitivity:0.056ng/ml
Kit Components:*152992A: Microtiter Strips, 1x48 wells or 1x96 wells, Pre-coated, ready to use*152992B: Standard, 2x1 vial152992C: Standard Diluent, 1x10ml or 1x20ml*152992D: Detection Reagent A (green), 1x60ul or 1x120ul*152992E: Detection Reagent B (red), 1x60ul or 1x120ul152992F: Assay Diluent A, 1x6ml or 1x12ml152992G: Assay Diluent B, 1x6ml or 1x12ml152992H: TMB Substrate, 1x4.5ml or 1x9ml152992K: Stop Solution, 1x3ml or 1x6ml152992L: Wash Buffer, 30X, 1x10ml or 1x20ml
Precaution:The Stop Solution (152992K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *152992A, *152992B, *152992D and *152992E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
仕様
Size：96Tests
Specificity：This assay has high sensitivity and excellent specificity for detection of Protamine 1 (PRM1). No significant cross-reactivity or interference between Protamine 1 (PRM1) and analogues was observed.
