Podoplanin (PDPN) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PDPN in mouse tissue homogenates, cell lysates and other biological fluids.
Detection Range:0.156-10ng/ml
Sensitivity:0.061ng/ml
Intra-Assay:CV<10%
Inter-Assay:CV<12%
Kit Components:*152962A: Microtiter Strips, 1x48 wells or 1x96 wells, Pre-coated, ready to use*152962B: Standard, 2x1 vial152962C: Standard Diluent, 1x10ml or 1x20ml*152962D: Detection Reagent A (green), 1x60ul or 1x120ul*152962E: Detection Reagent B (red), 1x60ul or 1x120ul152962F: Assay Diluent A, 1x6ml or 1x12ml1529626G: Assay Diluent B, 1x6ml or 1x12ml152962H: TMB Substrate, 1x4.5ml or 1x9ml*152962J: Positive Control, 0.98ng/ml, 1x1vial152962K: Stop Solution, 1x3ml or 1x6ml152962L: Wash Buffer, 30X, 1x10ml or 1x20ml
Precaution:The Stop Solution (152962K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *152962A, *152962B, *152962D, *152962E and *152962J at -20°C. After reconstitution of *152962J, store at 4°C and use within 5 days Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Notes on 152962J Positive Control:Intended Use:The control vials are from mouse sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
Characteristics:The control vials contain mouse PDPN in mouse sample (lyophilized powder). Note: The mouse based material used in this product is from healthy mice of SPF standard (specific pathogen-free animals).
Expected Values:0.98ng/mlThe concentration of the control was determined by subtracting two standard deviations to the mean optical density value of twenty QC sample replicates and calculating the corresponding concentration.
Usage:Disslove with 150ul standard dilutent and let stand for 10 minutes. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: After reconstitution, store Positive Control vial at 4°C and use within 5 days.
Assay Procedure Summary:1. Prepare all reagents, samples and standards;2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
6. Aspirate and wash 5 times
7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
